Isozymes
Isozymes have been most useful at the species level and below
Have been very useful to examine complex cases of hybridization and reticulate evolution because they are additive
e.g. have shown that hexapolid bread wheat (Triticum aestivum),contained sum of proteins possessed by ancestral species (T. dicoccum--a tetraploid + Aegilops squarrosa--a diploid)